OUP user menu

Analysis of SOX9 Expression in Colorectal Cancer

Bingjian MD, Yihu Fang MD, Jing Xu PhD, Lipei Wang PhD, Fangying Xu MD, Enping Xu PhD, Qiong Huang, Maode Lai MD
DOI: http://dx.doi.org/10.1309/AJCPW1W8GJBQGCNI 897-904 First published online: 1 December 2008

Abstract

Our purpose was to investigate the role of SOX9, a novel downstream molecule of β-catenin, in colorectal cancer. Expression of SOX9 and β-catenin was detected by immunostaining, quantitative real-time reverse transcription–polymerase chain reaction (Q-PCR), and Western blot in colorectal cancer. The correlation between SOX9 or β-catenin expression and clinicopathologic parameters was also analyzed. Immunostaining, Q-PCR, and Western blot consistently confirmed SOX9 up-regulation in colorectal cancer compared with normal mucosa (P < .05). Immunostaining showed more SOX9+ cells in the lower zone of colonic crypts than in the upper zone (P < .05). Cancers with strong SOX9 immunostaining were significantly associated with a lower 5-year overall survival (40% [17/43] vs low expression, 69% [66/95]; P <.01). The Cox proportional hazards model showed that strong SOX9 expression was an independent adverse prognosticator in colorectal cancer (P < .05). The detection of SOX9 expression might contribute to predicting clinical outcomes for patients with colorectal cancer.

Key Words:
  • Colorectal cancer
  • SOX9
  • β-Catenin
  • Prognosis