OUP user menu

Multiparameter Flow Cytometric Analysis Reveals Low Percentage of Bone Marrow Hematogones in Myelodysplastic Syndromes

Sepideh Maftoun-Banankhah MD, Atousa Maleki PhD, Nitin J. Karandikar MD, PhD, Arnaldo A. Arbini MD, Franklin S. Fuda DO, Huan-You Wang MD, PhD, Weina Chen MD, PhD
DOI: http://dx.doi.org/10.1309/4W2G3NDXUPG5J33N 300-308 First published online: 1 February 2008

Abstract

Diagnosis of myelodysplastic syndromes (MDS) could be difficult. We explored the usefulness of the enumeration of maturing B-lineage precursors (hematogones) by multiparameter flow cytometric analysis in the diagnosis of MDS in bone marrow (BM) specimens. We evaluated 111 MDS, 120 non-MDS (most with cytopenias; control group 1), and 41 noncytopenic lymphoma staging BM (control group 2) specimens. The percentage of total hematogones was significantly lower in MDS (median, 0%; mean, 0.10%) compared with non-MDS (control group 1, median, 0.38%, and mean, 0.91%; control group 2, median, 0.38%, and mean, 0.60%; P < .0001), as was the percentage of the most immature (stage I) hematogones. Thus, hematogone enumeration may serve as a biomarker to aid in the diagnosis of MDS. Interestingly, the percentage of hematogones was not significantly different between MDS subgroups or patients with MDS with and without chromosomal abnormalities, implying that a defect in maturing B-cell precursors may be an early event in the pathogenesis of MDS.

Key Words:
  • Hematogones
  • Myelodysplastic syndromes
  • Cytogenetics
  • Multiparameter flow cytometry