OUP user menu

VEGF Expression Correlates With Microvessel Density in Philadelphia Chromosome–Negative Chronic Myeloproliferative Disorders

Umberto Gianelli MD, Claudia Vener MD, Paola Rafaniello Raviele MD, Federica Savi MD, Francesco Somalvico, Rossella Calori MD, Alessandra Iurlo MD, Franca Radaelli MD, Elisa Fermo PhD, Paolo Bucciarelli MD, Silvano Bosari MD, Guido Coggi MD, Giorgio Lambertenghi Deliliersx MD
DOI: http://dx.doi.org/10.1309/FP0N3LC8MBJUFFA6 966-973 First published online: 1 December 2007


We examined microvessel density (MVD) and immunohistochemical expression of vascular endothelial growth factor (VEGF) in the bone marrow biopsy specimens of 98 patients with Philadelphia chromosome–negative (Ph–) chronic myeloproliferative disorders (CMPDs).

There were significantly more MVD “hot spots” in chronic idiopathic myelofibrosis (CIMF; mean ± SD, 25.6 ± 6.3) and polycythemia vera (PV; 20.7 ± 10.2) cases than in essential thrombocythemia (ET) cases (10.1 ± 4.5) and normal control (NC) samples (7.5 ± 3.6) (P < .05). Similar results were found using a semiquantitative method (P < .0001). A calculated VEGF index (VEGF(i)) was higher in CIMF (0.29 ± 0.15) and PV (0.28 ± 0.20) cases than in ET (0.12 ± 0.05) and NC (0.08 ± 0.04) cases (P < .0001). MVD and VEGF(i) were higher in the myelofibrotic phases of CIMF and PV. There was a direct correlation between VEGF(i) and MVD when considering the Ph– CMPDs together (r = 0.67; P < .001) and when considering PV (r = 0.79; P < .001) and CIMF (r = 0.40; P = .013) as individual entities.

Our data could provide a rationale for directly targeting VEGF or endothelial cells in CIMF and PV.

Key Words:
  • Vascular endothelial growth factor
  • VEGF
  • Microvessel density
  • Ph–chronic myeloproliferative disorders