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p16INK4A (CDKN2A) Gene Deletion Is a Frequent Genetic Event in Synovial Sarcomas

Manish M. Subramaniam MD, Rosa Noguera MD, Marta Piqueras, Samuel Navarro MD, Jose A. López-Guerrero PhD, Antonio Llombart-Bosch MD
DOI: http://dx.doi.org/10.1309/E2AAY2XXN431WL81 866-874 First published online: 1 December 2006


We assessed the frequency of genomic deletion of p16INK4A (CDKN2A) in synovial sarcomas (SSs) and its possible association with immunoexpression of p16 and cyclin D1 and the Ki-67 proliferation index using dual-color fluorescence in situ hybridization (FISH) on tissue microarray sections of 41 histologically and molecularly confirmed SSs. A heterozygous p16INK4A gene deletion was identified in 28 (74%) of 38 cases, with 25 (89%) of them showing abnormal p16 protein expression (20 negative and 5 heterogeneous). Of 25 cases, 19 (76%) exhibiting increased cyclin D1 expression also demonstrated heterozygous p16INK4A deletion. No significant association was observed between p16INK4A deletion and Ki-67 proliferation index, tumor grade, or histologic subtype. Our results demonstrate that p16INK4A (CDKN2A) gene deletion is a frequent genetic event in SS.

Key Words:
  • p16INK4A
  • Heterozygous deletion
  • Tissue microarray
  • Synovial sarcoma
  • Cell cycle