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Simple, Inexpensive, and Precise Paraffin Tissue Microarrays Constructed With a Conventional Microcompound Table and a Drill Grinder

Ulrich F. Vogel MD, Burkhard D. Bueltmann MD
DOI: http://dx.doi.org/10.1309/F2Q38DXN1V1V4GQM 342-348 First published online: 1 September 2006

Abstract

In 1998, paraffin tissue microarrays (PTMAs) as paraffin blocks containing up to 1,000 cylindrical paraffin tissue core biopsy specimens (PTCBs) for high-throughput molecular profiling of tumor specimens were introduced. PTCBs can be constructed using a manual tissue puncher/arrayer (Beecher Instruments, Sun Prairie, WI; cost, at least $7,000). Furthermore, custom-built PTMAs such as the MaxArray are created by companies such as Zymed Laboratories (South San Francisco, CA; PTMA with 96 holes, about $900). In our search for a less expensive alternative, we constructed PTMAs with up to 558 PTCBs by using a drill grinder, a drill stand, and a microcompound table (Proxxon, Niersdorf, Germany; cost, <$300).

Key Words:
  • Tissue microarrays
  • Technique
  • Microcompound table
  • Drill grinder
  • Paraffin tissue core biopsy specimens