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Risk Factor Analysis of Plasma Cytokines in Patients With Coronary Artery Disease by a Multiplexed Fluorescent Immunoassay

Thomas B. Martins, Jeffrey L. Anderson MD, Joseph B. Muhlestein MD, Benjamin D. Horne PhD, MPH, John F. Carlquist PhD, William L. Roberts MD, PhD, Harry R. Hill MD
DOI: http://dx.doi.org/10.1309/Q3E6KF0QD3U3YL6T 906-913 First published online: 1 June 2006

Abstract

Coronary artery disease (CAD) is the leading cause of death in the United States. Increasing evidence suggests involvement of inflammation in the atherosclerotic process. We examined cytokines and other inflammatory markers in 865 patients with chest pain in whom coronary angiography revealed no evidence of CAD or CAD with or without concomitant myocardial infarction (MI). We developed a multiplexed immunoassay to simultaneously assess the plasma concentrations of 8 cytokines (interferon γ, interleukin [IL]-2, IL-4, IL-6, IL-8, IL-10, IL-12, and tumor necrosis factor α), IL-2r, and soluble CD40 ligand in the patient groups. Concentrations of C-reactive protein (CRP) and IL-18 also were determined. Significant differences (P < .05) between no CAD and combined CAD groups were found for IL-2, IL-4, IL-6, IL-12, and IL-18. When the no CAD group was compared with the group with CAD with subsequent MI, significant differences were found for proinflammatory markers IL-6 (P ≤ .001), IL-8 (P = .017), and CRP (P ≤ .001). Cytokine profiles may have a role in differentiating patients with CAD with MI from those with chest pain due to other disorders and in deciphering the role of inflammation in the pathogenesis of CAD.

Key Words:
  • Inflammation
  • Atherosclerosis
  • Myocardial infarction
  • Cytokines
  • Multiplexed assay