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Immunophenotypic Profile of Lymphoplasmacytic Lymphoma/Waldenström Macroglobulinemia

Sergej Konoplev MD, PhD, L. Jeffrey Medeiros MD, Carlos E. Bueso-Ramos MD, PhD, Jeffrey L. Jorgensen MD, PhD, Pei Lin MD
DOI: http://dx.doi.org/10.1309/3G1XDX0DVHBNVKB4 414-420 First published online: 1 September 2005


We retrospectively reviewed the immunophenotypic profile of 75 cases of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM) analyzed by flow cytometry. All patients had monoclonal IgM (median, 2,100 mg/dL [21 g/L]) in serum and were considered clinically to have WM. The neoplastic cells, in all cases, expressed monoclonal immunoglobulin light chain (κ, 55;λ, 20) and CD19, and every case assessed was positive for CD20 (n = 68) and CD52 (n = 60). The results for other antigens assessed in decreasing frequency of positivity were as follows: surface IgM (26/28 [93%]), CD79b (11/13 [85%]), CD11c (13/16 [81%]), CD25 (5/7 [71%]), CD23 (17/28 [61%]), CD38 (24/50 [48%]), FMC7 (11/29 [38%]), CD22 (4/12 [33%]), CD5 (3/65 [5%]), and CD10 (1/38 [3%]). These results show that the immunophenotype of LPL/WM is variable and overlaps with other B-cell lymphoproliferative disorders. CD23, usually of dim intensity, and CD11c are expressed commonly in LPL/WM. Rare CD5+ and CD10+ cases of LPL/WM also exist.

Key Words:
  • Lymphoplasmacytic lymphoma
  • Waldenström macroglobulinemia
  • Flow cytometry
  • Immunophenotype