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Overexpression of Glutathione S-Transferase α in Clear Cell Renal Cell Carcinoma

Shang-Tian Chuang DO, Peiguo Chu MD, PhD, Jun Sugimura MD, Maria S. Tretiakova MD, PhD, Veronica Papavero MD, Kim Wang MD, MinHan Tan MD, Fan Lin MD, PhD, Bin T. Teh MD, PhD, Ximing J. Yang MD, PhD
DOI: http://dx.doi.org/10.1309/AQXR6B2QPUGD638C 421-429 First published online: 1 March 2005


To determine its diagnostic value, we evaluated glutathione S-transferase α (GST-α) expression in a large number of renal cell carcinomas (RCCs). GST-α messenger RNA (mRNA) levels from 70 renal neoplasms were analyzed with complementary DNA (cDNA) microarray chips containing 21,632 cDNA clones. Furthermore, 348 primary renal tumors and 24 metastatic RCCs were subjected to immunohisto-chemical analysis with a GST-α–specific antibody. GST-α mRNA was elevated significantly (11.4-fold) in a majority of clear cell RCCs (28/43 [65.1%]; 28/39 [71.8%] with adjustments for informative spots) compared with other kidney tumors (1/27 [3.7%]). Strong and diffuse GST-α immunoreactivity was demonstrated in a majority of clear cell (166/202 [82.2%]; mean intensity, 2.41) and metastatic clear cell RCCs (17/24 [70.8%]; mean intensity, 2.62). Other renal tumor types did not exhibit significant GST-α immunoreactivity, confirming mRNA results. Through cDNA microarrays and immunohistochemical analysis, we demonstrated GST-α as a biomarker for clear cell RCCs.

Key Words:
  • Glutathione S-transferase α
  • GST-α
  • Renal cell carcinoma
  • Complementary DNA microarray
  • Immunohistochemistry