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Flow Cytometric Immunophenotypic Characterization of Pediatric and Adult Minimally Differentiated Acute Myeloid Leukemia (AML-M0)

Patricia K. Kotylo MD, In-Sook Seo MD, Franklin O. Smith MD, Nyla A. Heerema PhD, Naomi S. Fineberg PhD, Kathy Miller MD, Marianne E. Greene MD, Pauline Chou MD, Attilio Orazi MD
DOI: http://dx.doi.org/10.1309/2FYJ-00BE-R8N0-HMPQ 193-200 First published online: 1 February 2000


We reviewed the clinicopathologic and immunophenotypic profiles of 7 pediatric and 11 adult minimally differentiated acute myelogenous leukemias (AML-M0). We also compared and evaluated myeloperoxidase in leukemic blasts using standard cytochemical and polyclonal antibody immunohistochemical stains. No distinctive clinical findings were noted in either patient group; however, thrombocytopenia typically was more prominent in adults. AdultAML-M0 also was associated with an immature myeloid profile (CD34+, terminal deoxynucleotidyl transferase positive, CD13+, and CD33+), in contrast with pediatric AML-M0, which usually lacked terminal deoxynucleotidyl transferase or CD34 but expressed bright CD33 with weak or negative CD13.Coexpression of the T-cell–associated antigen CD7 was observed in both groups. Antibody immunohistochemical stains were more sensitive than cytochemical stains for detection of myeloperoxidase activity and a useful adjunct for establishing a diagnosis of myeloid leukemia in paraffin-embedded marrow tissues.

Key Words:
  • Minimally differentiated acute myeloid leukemia
  • Pediatric
  • Adult
  • Immunophenotyping